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The RPE-choroid complex was dissected and permeabilized with 1% Triton for 12 h and then incubated in a blocking buffer (3% BSA, 0.3% Triton PBS) at room temperature for another 12 h

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Ultimately, the discussion now centers not only around the extent of regulation, but also on whether the EU can effectively demonstrate, through clear, transparent, and reproducible means, how scientific evidence informs policy decisions, consistent with its Better Regulation principles