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Real-time PCR was carried out in a C1000 Thermal Cycler from Bio-Rad (Hercules, CA) using SYBR Select Master Mix from Thermo Fisher Scientific per the included protocol

Family and friends: Protect your loved ones from passive smoking and be a positive role model for children

But before we dive into expiration mysteries, lets talk about how long your smokes are good for, what happens when cigarettes go stale, and how you can spot an ancient cigarette from miles away