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After incubation, the cells were washed three times with phosphate buffer saline (PBS, pH 5.8), followed by staining with Calcein Acetoxymethyl Ester (Calcein AM) and Propidium Iodide (PI) at 37 C for 30 min

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G., 1963, Distribution of a natural and an alien form of tobacco mosaic virus in the shoot apex of Nicotiana glauca Grah., Virology 21 : 300

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