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16S rRNA Gene Amplification and Sequencing Equal concentration of metagenomic DNA (~5 ng) was used for PCR amplification of bacterial 16S rRNA gene V3 hypervariable region

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Yach had done the unthinkable: accepting almost $1bn over 12 years from Philip Morris, makers of Marlboro and other leading brands, to set up his Foundation for a Smokefree World in New York in 2017 to fund research into alternatives to tobacco