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Step two is using it correctly

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The pre-filter requires monthly cleaning, while the other filters need to be replaced every six to eight months, depending on usage

Our previously described gating strategy [22] allows to define the cell numbers of alveolar macrophages (AM), conventional dendritic cell (cDC), interstitial macrophages (IMa), inflammatory monocytes (IMo), natural killer (NK), Natural killer T cells (, (NKT), CD4 and CD8 T cells (CD4 T and CD8 T) as well as their level of activation by the expression of I-Ab and CD86 expression for antigen presenting cells and of CD25 and CD69 for T lymphocytes