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[2] Clayton encouraged Harmon and his brothers to stay active in various sports before his sudden death in 1953 at age 59
Afterward, the obtained sperm pellets were treated with somatic lysis buffer (SLB: 0.1% SDS, 0.5% Triton-X-100 in DEPC water) on ice for 20 min and then centrifuged at 1000 g for 5 min
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Assess blood pressure before initiating treatment with ZYBAN, and monitor periodically during treatment
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