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Cells were viewed with a Nikon Eclipse Ti inverted microscope (Nikon Instruments, Melville, NY, USA) using a 60X objective, and images were collected using an Andor Zyla VSC-04941 camera (Andor, Belfast, UK) at exposures that did not produce saturation

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Consider professional cleaning for heavily affected pieces

[Easy to Clean & Maintain]:Built with your convenience in mind, this pod coffee maker features a descaling reminder, which will auto-prompt and help you to clean up the k cup coffee machine after 300 brews