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Method of metabolomics detection and analysis For lung tissue homogenate samples, 400 l MeOH (containing 100 ng/mL Warfarin as Internal Standard) was added to the samples (100 l), which were then vortexed for 60 s, followed by centrifugation at 14,000 g and 4 for 15 min

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Hold the end with the blue line up, snap it to release the alcohol to the other end, and swab away any tough residue