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They then observed that when the cells treated with nicotine were subsequently exposed to environmental causes of mutation, such as UV rays, the nicotine-exposed cells had between two and four times more spontaneous mutations, suggesting that the nicotine predisposed cells to mutation from other sources

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Main effects of group and time, group-by-time interaction and partial 2 ( 2 0.14: large effect) (Cohen 2013) as effect size were calculated