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CM 1900 Cryostat, Leica Biosystems) were thaw-mounted onto indium tin oxidecoated glass slides and dried, and 2,5-dihydroxybenzoic acid (DHB) (40 mg/mL, 70% methanol with 0.1% trifluoroacetic acid) was applied (TM Sprayer, HTX Technologies)

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Smoking is one habit many Americans seem to have dropped -- or never picked up -- in the last few decades

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