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To study the effects of denicotinized cigarettescigarettes from which nicotine has been removedon a specific type of nAChR (a4b2* nAChRs), researchers funded in part by NIDA used a brain imaging technique known as positron emission tomography (PET) to visualize these receptors in the brains of smokers during withdrawal and after smoking either low-nicotine or denicotinized cigarettes

Eluted protein samples were filtered and injected into an autosampler-equipped KTA pure system on a Superdex S75 Increase 10/300 GL column at room temperature using SEC running buffer (20 mM Tris-HCl and 100 mM NaCl (pH 8))