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After the 48 h, the supernatant was removed and a 25 mM of culture medium containing 0.5 mg/mL of MTT salt solution was added to each well and it was again adjusted in a 5% carbon dioxide incubator at 37 C for 4 h
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Early on it was believed that chemical mutation within the p53 gene probably occurred at random and that selective growth advantages associated with certain forms of mutated p53 encouraged lung tumor expansion (8)
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