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Over time, the tobacco market has become more global (Jha and Chaloupka 2000) due to loosened trade restrictions, rising incomes in many countries, and aggressive marketing by tobacco companies

On December 20, 1952, a further gift of another 8 acres adjoining the original tract was made to the Arboretum - 4 acres were meadowland, intended for the planting of additional trees (native to the area as well as rare and unusual specimens.) The other 4 acres already had many trees and wildflowers and some shrubs growing on them

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Nevertheless, there is limited knowledge regarding alterations in the oral microbiome throughout gestation and the potential impact of pregnancy-induced changes on outcomes 4,5,6