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Essentially, the new guidelines call for clinicians to stop directing patients to start taking a medication a few days before quitting

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Proteins were separated in 1 MES running buffer (Bio-Rad) by electrophoresis (100130 V for 1 h), whereafter proteins were transferred from the gel to a 0.45 M Nitrocellulose Transfer membrane (Bio-Rad) by electroblotting (100 V for 1 h) using a Bio-Rad Criterion Blotter

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