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2.8 Semi-native gel electrophoresis 5-L reaction samples or 10 g of purified VLPs were supplemented with 6x gel loading purple dye (NEB) prior to separation by 1% (w/v) agarose gel electrophoresis for 60 min at 60 V in TAE buffer (40 mM Tris base, 20 mM acetic acid, 1 mM EDTA) supplemented with 0.3 g/mL ethidium bromide (Roth)

The totals from (F) were subtracted from the numbers counted in the targeted suite of analyses (E) to establish the numbers of compounds detected only in the targeted suite

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