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Nicotine self-administration in rats: strain and nicotine pre-exposure effects on acquisition

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At present, it is impossible to reach a consensus on the safety of e-cigarettes except perhaps to say that they may be safer than conventional cigarettes but are also likely to pose risks to health that are not present when neither product is used, wrote physicians Chitra Dinakar and George OConner in The New England Journal of Medicine in 2016

Animal study design Adult female sheep (ewes) were used in all experiments because male sheep are naturally more aggressive and thus not amenable for long-term experimentation without the use of general anesthesia

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