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Detached cells were transferred to flow cytometry tubes and centrifuged at 1500 rpm at room temperature

You wont have to worry about the hassle of setting anything up or getting something complex ready before you inhale

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Downregulation of HIF-1 is in good agreement with the lack of upregulation of glycolysis in our study but is in contrast to HIF-1 regulation reported in orbital fibroblasts, where HIF-1 protein levels and HIF signaling were both upregulated by CSE treatment [37]