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Similarly, HAEC cells were cultured in 6-well plates at a density of 2.0 10 5 cells/well, starved overnight, and treated with 50 mg/ml ENDS liquid with nicotine or ENDS liquid without nicotine for 24 hours or 1 mM H 2 O 2 for 12 hours (positive control) and then subjected to Annexin V/PI apoptosis analysis by flow cytometry
Some reactions were accompanied by cardiovascular collapse, acute myocardial ischemia with or without myocardial infarction, loss of consciousness, tingling, pharyngeal or facial edema, dyspnea, urticaria, and itching
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