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Interestingly, in the frontal cortex, hippocampus, striatum, and cerebellum, chronic nicotine administration was shown to upregulate CYP2D6 mRNA in male rats (169) and CYP2D mRNA and protein in African green monkeys (170)

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Equal amounts of proteins were electrophoresed on a 10% SDS-PAGE gel and then transferred to a polyvinylidene difluoride membrane that had been activated with methanol

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