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One study, for example, treated Sprague Dawley rats treated with gestational nicotine (3 mg/kg/day) and found that PNE offspring (P35/P36) had altered myelin expression of myelin-related transcription and trophic factors that produced alterations in myelin gene expression and oligodendrocyte functioning [165]

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Tapering phase (weeks 13 onward) At this end-of-treatment visit, participants will provide a urine sample to assess urinary anabasine (a marker of smoking in the presence of NRT) [37]