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Next, TP (100 g, KRK26) was weighed into Tissue Culture Bottles, followed by spraying 40 mL bacterial suspension evenly to the sample under stirring, and fermentation at 37 C for 7 days (Dai et al

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The study found that protease levels (such as neutrophil elastase, MMP-2 and MMP-9) were elevated in both e-cigarette users and smokers, similar to those in smokers, while anti-protease levels remained unchanged