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Permeability measurements were reported as percentage of controls (the permeability coefficients of controls were as follow: FITC 0.198 0.009 10 3 cm/min
Briefly, SV-HUC cells were plated in 24-well plates (2 10 4 /well) and treated twice per week with EV-depleted complete culture medium containing 20 g/mL of BCEVs isolated from TCCSUP cells that were untreated or were treated with UEL, MEL, or CSE (8 replicate wells per treatment condition)
The problem can be compounded when smokers are also in bad health, especially from smoking-related diseases such as high blood pressure or emphysema
In our study, some participants have attributed their relapses to seeing their friends smoke, and have stated that being surrounded by smokers is a major barrier for them to seek tobacco cessation
