We then specifically tested the hypotheses that optogenetic activation of VTA DA neurons should reproduce the increased exploitation seen in nicotine-treated animals, and that optogenetic inhibition should produce the opposite effect
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drooling, nausea, vomiting, diarrhea, unsteady gait, dilated pupils, agitation, nervousness, weakness, an abnormal heart rate, high blood pressure, panting, tremors, seizures, paralysis, respiratory arrest, and even death