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Studies in mice investigating the effects of e-cigarettes on different brain regions have found that e-cigarette exposure during pregnancy can alter the transcriptome of the frontal cortex (1316 mg/mL nicotine), increase global DNA methylation within the brain (1316 mg/mL nicotine), and dysregulate gene expression in the hippocampus (13 mg/mL nicotine) [176]

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The difference between safe use, like with nicotine patches or gum, and overdose depends on how much nicotine youre exposed to and how it enters your body (e.g., ingesting via drinking, skin, smoking)

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However, you may need to refrain from medications that require you take them with food