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The RPE-choroid complex was dissected and permeabilized with 1% Triton for 24 h, After blocking, samples were incubated overnight at 4 C with a mixture of anti-Sema4D (Abcam, #ab231961, 1:50), anti-PlexinB1 (Santa Cruz, #sc-28372, 1:20), and anti-CD8 (Abcam, #ab308264, 1:50) antibodies in blocking solution
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Plasma-free fatty acids, fatty acid-binding protein 4, and mortality in older adults (from the Cardiovascular Health Study)