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We investigated with voltage-clamp experiments, effects of aqueous extracts (coffees) of unroasted and roasted coffee beans on the activity of human brain nicotinic acetylcholine receptor (nAChR) subtypes expressed in Xenopus oocytes, looking at complex brews, low molecular weight (LMW) fractions, and specific compounds present in coffee

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Surprisingly, we observed no significant transcriptional differences in other mitochondrial fission (Fis1 and Drp1) or fusion markers (Mfn1 and Mfn2) nor in Tfam expression (Figure 2)