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The recommended dietary allowance for adults is 2.4 micrograms per day according to the NIH Office of Dietary Supplements (updated 2025)

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Its important to note that early detection and treatment of Vitamin B-12 deficiency is vital because if left untreated, the deficiency can cause severe neurologic problems and blood diseases

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The animals were kept in a controlled environment with stable temperature and humidity under a 12-hour light/dark cycle