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Caspase-3 activity assay Both hepatocytes and liver tissues were lysed on ice with lysis buffer containing protease inhibitors, and the proteins were collected

That led us to wonder whether a compound with strong antioxidant capacity might protect HGF, either by preventing nitration or by compensating for the functional loss it causes. The team turned to two sulfur-based compounds known for strong antioxidant activity: glutathione trisulfide (GSSSG) and lipoic acid trisulfide (LASSS)