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None of the three compounds have safety data in this context

CIT, classified as a non-essential amino acid and a precursor to L-arginine, has been shown to facilitate the production of NO and promote vasodilation, consequently enhancing endothelial functionality and muscle perfusion (911)

They provide the clinical context that no test can replace

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The Lipofectamine 3000 Kit (L3000015, Thermo Fisher Scientific) was used to transfect the cells with two plasmids: the 2.5 g negative control plasmid pcDNA3.1 and the 2.5 g pcDNA3.1 MPO plasmid