Primary antibodies were diluted in TBST skimmed milk 5% buffer and the incubation was performed overnight at 4C using an orbital shaker followed by 2 h at room temperature with the secondary horseradish peroxidase (HRP)-linked antibody (Goat Anti-Mouse or Anti-Rabbit IgG Peroxidase Conjugated by Thermo Scientific)
A healthy body maintains a high ratio of reduced glutathione to oxidized glutathione
Minhas AK, Hodgson P, Barrow CJ, Adholeya A
Holmes HJ, Thakur ER, Carty JN, Ziadni MS, Doherty HK, Lockhart NA, et al
Importance dans les domaines humains et industriels Dans le corps humain, le glutathion est abondant dans des organes tels que le foie et joue un rle cl dans la dfense antioxydante, la dtoxification de phase II et le maintien des voies de signalisation sensibles l'oxydorduction, ce qui a engendr une forte demande de GSH de qualit pharmaceutique et nutraceutique