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Materials and Methods Fluorescent instrumentation Perkin-Elmer fluorescent spectrometer model 1000 set at excitation wavelength 475nm and its scale expansion gain control knob was adjusted so that a 10M solution of the bimane conjugate gave a full scale reading of 100 fluorescence units

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doi: 10.1111/j.1349-7006.2010.01684.x 134 KuligPBurkhardSMikita-GeoffroyJCroxfordALHvelmeyerNGylvsziGet al