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MOTS-c activates AMPK to shift cellular metabolism toward fat oxidation and improve insulin sensitivity, while 5-Amino-1MQ inhibits NNMT to preserve NAD+ availability and enhance mitochondrial function

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When PI3K and its regulatory proteins, p85 and p110, are recruited by IRS, they will further recruit and activate PDK1 (PIP 3 -dependent kinase 1), Akt (PKB), mTORC2, S6 kinases and PKC