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While common adverse effects such as gastrointestinal symptoms are well documented ( Statements Data availability statement The original contributions presented in the study are included in the article/supplementary material, further inquiries can be directed to the corresponding author

The expression of each gene of interest (Supplementary Table 1) was assessed using the Comparative CT method employing SYBR green master mix (Invitrogen Cat #4309155)

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Aggregation rather than shear is the specific risk with an amylin-family sequence: the prolines at positions 25, 28 and 29 reduce fibrillation propensity relative to native amylin but do not eliminate it, so inspect the solution against a light source for haze, opalescence or filaments before use