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The viruscontaining supernatant was collected 48 hrs after transfection and passed through a 0.22 m filter to eliminate cells

Apparatus and chromatographic conditions

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This heterogeneity reflects the intermediate position of this peptide, which is neither an approved medication nor a strictly controlled substance in most countries

briefly we put three A sheets (control) and two B sheets (intervention) in an envelope and each time we randomly picked one of the sheets and assigned the patient to the control or intervention groups, respectively