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Risks: Includes nasal irritation, inconsistent plasma concentration, and off-label concerns

10.1016/j.mce.2014.08.014 72 HuangL.YaoT.ChenJ.ZhangZ.YangW.GaoX.et al (2022d)

The protein was eluted in three steps, first with 5 ml of elution buffer containing 50 mM imidazole (50 mM NaH 2 PO 4 , 300 mM NaCl and 50 mM imidazole, pH 8.0) and then with 5 ml of elution buffer containing 100 mM imidazole and finally with 5 ml of elution buffer containing 500 mM of imidazole

Diabetologia 53(4):730740 Marx N, Burgmaier M, Heinz P, Ostertag M, Hausauer A, Bach H, Durst R, Hombach V, Walcher D (2010) Glucagon-like peptide-1(137) inhibits chemokine-induced migration of human CD4-positive lymphocytes