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I consistently recommend completing at least the minimum protocol duration even when subjective improvement comes early

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Our lysis buffer contained 50 mM Tris-HCl, pH 7.5, 250 m NADP + (for G6PD stabilization) 5% (v/v) glycerol, 0.5 l/ml -mercaptoethanol, 150 mM NaCl, 0.1% Triton X-100, 1 mM Pefabloc SC, and 1% protease inhibitor cocktail (for use with plant extracts, Sigma)

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