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For example [42], showed that chronic cadmium exposure led to upregulated of MMP9 in rat lung tissue

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ROC analyses were completed to identify cut-points for severity thresholds between anchor categories, including a) the whole population (e.g., No to Moderate versus Severe) or b) only individuals in adjacent categories (e.g., Moderate versus Severe) to estimate the threshold, first at all timepoints in a stacked dataset and then split by timepoint (Baseline/Post-Baseline)