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Full reduction of the probe through incubation of seedlings with 10 mM DTT caused only a small drop in the detected fluorescence ratio
In fact, it's so important that it's often referred to as the "master antioxidant"
The 1 st incubation wells (ACSF + inhibitors) were aspirated (bottom of the well (~500 L)) with a pipette
[42] [43] Liraglutide was approved by the FDA in 2019, for treatment of children aged ten years of age or older with type 2 diabetes, making it the first non-insulin drug approved to treat type 2 diabetes in children since metformin was approved in 2000
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