The trial proceeded without reported toxicity, and no lethal dose threshold (LD1) was ever established in subsequent safety testing meaning researchers administering the compound could not find a dose that caused death in even 1% of test subjects across the animal model safety work that preceded and followed the human study
The extracted details, such as authors, study population, study design, definition of smoking status, median follow-up time and outcomes, were systematically documented and organised in a table
To measure oxidative stress, the study evaluated nine different biomarkers: ammonia, beta-carotene, glutamine, glutathione, malondialdehyde, total antioxidant status (TAS), vitamin C, vitamin E-alpha tocopherol, and vitamin E-gamma tocopherol
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