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Age significantly influences achievable improvement magnitude with younger users (20s-30s) typically experiencing more dramatic results as their skin retains better responsiveness to peptide stimulation and baseline collagen production remains moderate rather than severely diminished

The original mouse study used roughly 3 weeks of alternating-day treatment

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Under a non-monotonic curve, an increase producing less effect is ambiguous: it cannot distinguish being below the peak from having passed it

The peptide enhances healing but cannot override continued tissue damage from excessive mechanical stress