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Whole blood was drawn by cardiac puncture under sterile conditions into CPDA-1, was centrifuged, and the hematocrit was adjusted to 75% by removing supernatant

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The mobile phase contained ammonium dihydrogen phosphate (75 mM), and the pH was adjusted to 5.7 using ammonia solution, which was then degassed in an ultrasonic bath for 10 min at room temperature

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