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Epitomics), The membranes were washed three times in TBST for 5 mins each and were incubated at room temperature for 1 hr with horseradish peroxidase conjugated rabbit anti-mouse or anti-rabbit IgG secondary antibody (Sigma-Aldrich, anti-mouse Cat

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This hormone has several key functions: it stimulates insulin release from the pancreas when blood sugar levels are high, inhibits glucagon secretion (which raises blood sugar), slows down gastric emptying, and influences brain signals related to satiety

When thirst occurs, it is typically an indirect consequence of gastrointestinal side effects like vomiting or diarrhea that cause dehydration