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We used ddPCR Supermix for probes (Bio-Rad product number 186-3010), an automated droplet generator and droplet reader (Bio-Rad QX200), and PCR cycling conditions according to the manufacturers instructions as follows: enzyme activation 95 C for 10 min (one cycle), denaturation 94 C for 30 s followed by annealing/extension at 60 C for 1 min (40 cycles), enzyme deactivation 98 C for 10 min (one cycle), and hold at 4 C, as previously described 19

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