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The liver stores 50 to 90% of the bodys total B12 reserves

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Iron overload has also been observed in rare cases of children with inborn brain neurodegeneration due to iron accumulation (NBIA) associated with autism-like conditions (Zigman et al., 2021) and anxiety-like behaviors (Maaroufi et al., 2009), although the causal relationship remains obscure

To check the effect of an oxidant (H 2 O 2 ) or anti-oxidant (NAC), all culture media were replaced with fresh medium containing H 2 O 2 or NAC on day 8