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HPLC and mass spectrometry were carried out following previous methods, with modifications ( Monoamine oxidase A activity, qRT-pCR and dopamine content ELISA kit The striatum of mice was excised with a mold in a buffer solution at 4C, quickly frozen in liquid nitrogen, and stored at 80C

Implications A natural extension of the present work for future research would be to test the convergence of the substitute products for cigarettes, which would give an answer as to whether the greatest convergence observed from 2010 is related to the behavior of other substitute products, which include legal tobacco products, such as fine-cut tobacco or pipe tobacco, and even illegal products

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